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cyclin d1 cell signaling technology 2978s wb  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cyclin d1 cell signaling technology 2978s wb
    Cyclin D1 Cell Signaling Technology 2978s Wb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1851 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclin d1 cell signaling technology 2978s wb/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1851 article reviews
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    cyclin d1 cell signaling technology 2978s wb  (Cell Signaling Technology Inc)
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    Cyclin D1 Cell Signaling Technology 2978s Wb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    cyclin d1 92g2 cell signaling technology wb  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc cyclin d1 92g2 cell signaling technology wb
    Figure 3 Silencing ARID1A promotes NPC cell proliferation, migration and invasion. (A) According to Western blot assay results, ARID1A was successfully knocked down by siRNA in CNE1 and HNE1 cells, which also resulted in <t>Cyclin</t> <t>D1</t> upregulation. (B) The effects of ARID1A silencing on cell proliferation were measured by CCK8 assay. (C) Cell cycle distribution was analyzed by flow cytometry at 48 hrs post-culture. (D) Relative cell distribution percentage of ARID1A- silenced (si-ARID1A) and control (si-ctrl) NPC cells in each cell cycle phase. (E and F) Motile and invasive activities of the si-ARID1A and si-ctrl groups of NPC cells based on transwell assays. The average number of cells per field was determined from three repeated independent experiments. *P<0.05, **P<0.01, *** or #P<0.001. Abbreviations: NPC, nasopharyngeal carcinoma; ARID1A, AT-rich interacting domain-containing protein 1A.
    Cyclin D1 92g2 Cell Signaling Technology Wb, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Figure 3 Silencing ARID1A promotes NPC cell proliferation, migration and invasion. (A) According to Western blot assay results, ARID1A was successfully knocked down by siRNA in CNE1 and HNE1 cells, which also resulted in Cyclin D1 upregulation. (B) The effects of ARID1A silencing on cell proliferation were measured by CCK8 assay. (C) Cell cycle distribution was analyzed by flow cytometry at 48 hrs post-culture. (D) Relative cell distribution percentage of ARID1A- silenced (si-ARID1A) and control (si-ctrl) NPC cells in each cell cycle phase. (E and F) Motile and invasive activities of the si-ARID1A and si-ctrl groups of NPC cells based on transwell assays. The average number of cells per field was determined from three repeated independent experiments. *P<0.05, **P<0.01, *** or #P<0.001. Abbreviations: NPC, nasopharyngeal carcinoma; ARID1A, AT-rich interacting domain-containing protein 1A.

    Journal: Cancer Management and Research

    Article Title:

    Loss of ARID1A promotes proliferation, migration and invasion via the Akt signaling pathway in NPC

    doi: 10.2147/cmar.s207329

    Figure Lengend Snippet: Figure 3 Silencing ARID1A promotes NPC cell proliferation, migration and invasion. (A) According to Western blot assay results, ARID1A was successfully knocked down by siRNA in CNE1 and HNE1 cells, which also resulted in Cyclin D1 upregulation. (B) The effects of ARID1A silencing on cell proliferation were measured by CCK8 assay. (C) Cell cycle distribution was analyzed by flow cytometry at 48 hrs post-culture. (D) Relative cell distribution percentage of ARID1A- silenced (si-ARID1A) and control (si-ctrl) NPC cells in each cell cycle phase. (E and F) Motile and invasive activities of the si-ARID1A and si-ctrl groups of NPC cells based on transwell assays. The average number of cells per field was determined from three repeated independent experiments. *P<0.05, **P<0.01, *** or #P<0.001. Abbreviations: NPC, nasopharyngeal carcinoma; ARID1A, AT-rich interacting domain-containing protein 1A.

    Article Snippet: 89 .1 00 .1 11 o n 20 -J un -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Table S2 List of antibodies and suppliers used for immunohistochemistry or immunoblotting Antibody Clone Suppliers Application Dilutions ARID1A PSG3 SANTA CRUZ BIOTECHNOLOGY WB 1:200 IHC 1:100 Akt1 B-1 SANTA CRUZ BIOTECHNOLOGY WB 1:200 E-cadherin 67A4 SANTA CRUZ BIOTECHNOLOGY WB 1:200 Vimentin 92G2 SANTA CRUZ BIOTECHNOLOGY WB 1:200 P-Akt1 104A282 SANTA CRUZ BIOTECHNOLOGY WB 1:200 IHC 1:100 Cyclin D1 92G2 Cell Signaling TECHNOLOGY WB 1:1,000 β-Actin OTI1 ZSGB-BIO WB 1:1,000 0hA B C 24h 48h 0h 24h 48h si -c trl si -A R ID 1A si -c trl H N E 1 C N E 1 si -A R ID 1A 24 h ga p cl os ur e (% ) 48 h ga p cl os ur e (% ) si-ctrl 40 30 20 10 0 CNE1 HNE1 80 60 40 20 0 CNE1 HNE1 si-ARID1A si-ctrl si-ARID1A Figure S2 ARID1A knockdown promoted cell migration in NPC cells by wound scratch assays.

    Techniques: Migration, Western Blot, CCK-8 Assay, Cytometry, Control

    Figure 7 MK-2206 rescued the changes in Cyclin D1 and EMT-associated markers in ARID1A-silenced NPC cells. Western blot analysis of the expression of ARID1A, p-Akt, Akt, Cyclin D1, E-cadherin and Vimentin. MK-2206 rescued the changes in Cyclin D1, E-cadherin and Vimentin expression in ARID1A-silenced NPC cells. Abbreviations: NPC, nasopharyngeal carcinoma; ARID1A, AT-rich interacting domain-containing protein 1A.

    Journal: Cancer Management and Research

    Article Title:

    Loss of ARID1A promotes proliferation, migration and invasion via the Akt signaling pathway in NPC

    doi: 10.2147/cmar.s207329

    Figure Lengend Snippet: Figure 7 MK-2206 rescued the changes in Cyclin D1 and EMT-associated markers in ARID1A-silenced NPC cells. Western blot analysis of the expression of ARID1A, p-Akt, Akt, Cyclin D1, E-cadherin and Vimentin. MK-2206 rescued the changes in Cyclin D1, E-cadherin and Vimentin expression in ARID1A-silenced NPC cells. Abbreviations: NPC, nasopharyngeal carcinoma; ARID1A, AT-rich interacting domain-containing protein 1A.

    Article Snippet: 89 .1 00 .1 11 o n 20 -J un -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Table S2 List of antibodies and suppliers used for immunohistochemistry or immunoblotting Antibody Clone Suppliers Application Dilutions ARID1A PSG3 SANTA CRUZ BIOTECHNOLOGY WB 1:200 IHC 1:100 Akt1 B-1 SANTA CRUZ BIOTECHNOLOGY WB 1:200 E-cadherin 67A4 SANTA CRUZ BIOTECHNOLOGY WB 1:200 Vimentin 92G2 SANTA CRUZ BIOTECHNOLOGY WB 1:200 P-Akt1 104A282 SANTA CRUZ BIOTECHNOLOGY WB 1:200 IHC 1:100 Cyclin D1 92G2 Cell Signaling TECHNOLOGY WB 1:1,000 β-Actin OTI1 ZSGB-BIO WB 1:1,000 0hA B C 24h 48h 0h 24h 48h si -c trl si -A R ID 1A si -c trl H N E 1 C N E 1 si -A R ID 1A 24 h ga p cl os ur e (% ) 48 h ga p cl os ur e (% ) si-ctrl 40 30 20 10 0 CNE1 HNE1 80 60 40 20 0 CNE1 HNE1 si-ARID1A si-ctrl si-ARID1A Figure S2 ARID1A knockdown promoted cell migration in NPC cells by wound scratch assays.

    Techniques: Western Blot, Expressing